Open AccessPathomorphological changes in the testicles of goats in acute anaplasmosis
Author(s) -
V. Mihaylenko,
Olga Dilekova,
Sergey Kartashov,
V. Ya. Meshcheryakov,
В. М. Шпыгова,
Nikolay Agarkov
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/403/1/012061
Subject(s) - pathology , necrosis , vacuolization , coagulative necrosis , infiltration (hvac) , biology , lumen (anatomy) , haematoxylin , anatomy , staining , medicine , physics , thermodynamics
The aim of the research was to study pathomorphological changes in the testicles of goats in acute anaplasmosis. Diagnosis of anaplasmosis in sick goats was carried out taking into account the characteristic clinical signs and studies of peripheral blood smears. Blood smears after fixation in alcohol ether were stained according to Romanovsky-Giemsa by hematoxilin and eosin. For histological examination, pieces of testicles and appendages of testicles of 0.5 cm thick were selected, which were fixed in an 8% neutral aqueous solution of formalin. The resulting material was poured into paraffin according to the generally accepted method. Histological sections of 5-8 microns thick were made from the obtained paraffin blocks and stained with hematoxilin and eosin using the method of Van Gieson and Mallory. Micro photos were made with a series of rendered images on the basis of Olimpus 2000. The study revealed the following changes: the spermatogenic epithelium has desquamation, in the lumen of the convoluted seminiferous, there were tubules protein detritus, exfoliated cells of seminiferous epithelium and macrophages. Sertolli cells were in a state of vacuole dystrophy and necrosis. In the interstitial tissue around arterial vessels and convoluted seminiferous tubules, focal accumulation of lymphoid-macrophage infiltration was found. Endocrine cells located in the interstitial were in a state of vacuole dystrophy and necrosis. Thus, in goats older than 6 months with acute anaplasmosis in the testicles, pathomorphological changes are characteristic of acute parenchymal orchitis.