Open AccessCharacterization of Peanut Stripe Virus from West Nusa Tenggara
Author(s) -
Nur Indah Julisaniah,
Suharjono Suharjono,
Retno Mastuti,
Estri Laras Arumingtyas
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/391/1/012043
Subject(s) - arachis hypogaea , genbank , biology , coat protein , chenopodium quinoa , chenopodium , coat , plant virus , botany , virus , gene , virology , rna , biochemistry , paleontology , weed
Peanut Stripe Virus (PStV) infection on peanut plants causes a reduction in the yield of peanuts. This study was aimed to characterize PStV that infected peanut ( Arachis hypogaea ) plants in the West Nusa Tenggara province. Peanut plants that showed symptoms of stripes on leaves were collected from West Nusa Tenggara province peanut fields. Stripes symptoms on leaves indicated that the plant was infected with PStV. PStV mechanical inoculation to the healthy Chenopodium amaranticolor plant with sap from PStV infected peanut leaf showed local lesions symptoms on the leaf. Molecular characterization was carried out using RT-PCR with specific primers produced fragments of 1071 nt, consists of NIb, coat protein (CP) gene, and 3’UTR. Based on nucleotide sequences of coat protein gene, PStV from West Nusa Tenggara 98-99 % was similar with others PStV isolated reference in Genbank.