Open AccessThe effect of nitrogen sources on anti-phytopathogenic activities fermented filtrate of Bacillus subtilis AAF2
Author(s) -
Syukria Ikhsan Zam,
Oksana,
Anthoni Agustien,
Syamsuardi,
Akmal Djamaan,
Irfan Mustafa
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/391/1/012005
Subject(s) - sclerotium , bacillus subtilis , fusarium oxysporum , carbon source , nitrogen , fermentation , yeast extract , food science , chemistry , corn steep liquor , biology , botany , microbiology and biotechnology , horticulture , bacteria , biochemistry , organic chemistry , genetics
The filtrate from Bacillus subtilis AAF2 fermentation was reported to have high anti-phytopathogenic activity in the corn steep liquor modified medium with glucose as its carbon source. Besides media and carbon sources, the nitrogen source also affects these activities. This study aims to obtain the best nitrogen source on anti-phytopathogenic activities fermented filtrate of B. subtilis AAF2. The nitrogen sources used were yeast extract (15 G L -1 ), peptone (15 G L -1 ), NH 4 NO 3 (1.71 G L -1 ), and (NH 4 ) 2 SO 4 (2.83 G L -1 ), with phytopathogen test were Fusarium oxysporum and Sclerotium rolfsii . The parameters measured were pH fluctuations, the number of B. subtilis AAF2 cells, and the efficacy of phytopathogen growth inhibition (measured every 24 hours for 3 x 24 hours). The results showed pH ranged from 5.7 to 7.1; the number of cells ranges from 2.1X10 6 -1.00X10 15 CFU ml -1 ; the efficacy against F. oxysporum ranged from 7.10 -71.40% and against S. rolfsii ranged from 17.70 -71.80%. The best nitrogen source in the fermentation of anti-phytopathogenic compounds by B. subtilis AAF2 was peptone.