Open AccessBiodegradation of (N-phosphonomethyl)glycine Utilizing Bacillus subtilis using different incubation periods
Author(s) -
Nibal Kh. Mousa,
I. H. Gatie,
A. K. M. Mahbub Hasan
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/388/1/012080
Subject(s) - bacillus subtilis , incubation , glyphosate , bioremediation , biodegradation , glycine , chemistry , food science , incubation period , high performance liquid chromatography , phosphorus , bacillales , chromatography , biology , zoology , environmental chemistry , contamination , bacteria , biochemistry , agronomy , ecology , organic chemistry , amino acid , genetics
The study aimed to evaluat Bacillus subtilis biodegrdation one of organic-pesticides in different periods. Bacillus subtilis was isolated from Iraqi soils, identification by morphological and biochemical tests. The best growth of B. subtilis were in(72hours) / (10-15) ppm (0.200-0.196)respectively, while the 5 ppm showed the highest growth in 60 day (0.163). The best degradation rate% were for 15 ppm/14 days (90.32)%. The evluation of (N-phosphonomethyl)glycine residues concentration’s via HPLC and degradation ratio%, showed with increasing time incubation to 30 days, Bacillus subtilis degradation ratio% increased for (15)ppm, while the best 60 days / (25)ppm. From all the conclusion is that the B. subtilis used the Glyphosate as source for carbon and phosphorus and suggest could be well exploited for bioremediation of Glyphosate contaminated sites in 15ppm/30 days and 20 ppm/60 days.