Open AccessAgrobacterium mediated transformation of Banten local rice (cv. Pare Gajah) for Folate gene
Author(s) -
Susiyanti Susiyanti,
Tamara Larasati,
Nurmayulis,
Sulastri Isminingsih,
Sjaifuddin,
suseno amin
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/383/1/012046
Subject(s) - transformation (genetics) , explant culture , agrobacterium , agrobacterium tumefaciens , horticulture , biology , in vitro , transformation efficiency , microbiology and biotechnology , botany , gene , genetics
Folate (vitamin B9) deficiency is a common problem that often occurs in developing countries including Indonesia. Rice which is a staple containing folate is very low. So it is necessary to find an effort so that the rice consumed can contain folate which is good for health.The aim of this study was to transform the folate gene to plant Banten local rice ( cv . Pare Gajah) using Agrobacterium tumefaciens . This research was conducted from January to Mei 2019 in the Laboratory of Physiological and Plant Biotechnology, Agriculture faculty, Sultan Ageng Tirtayasa University. Transformation research used a Completely Randomized Design with two factors, the first factor is the transformation method (I) consist of 2 levels; namely in vitro and in planta. The second factor is hygromycin concentration (H) consist of 4 levels; namely 25, 50, 75, and 100 ppm. Each treatment was replicated six times. The results showed, in vitro transformation method had a lower lethal dose, causing average death of explant reaches 93,75%. In planta transformation method giving the best average efficiency transformation reach 13,33%. Hygromycin concentration 100 ppm causing the higher average death of explant reaches 98,34%. Hygromycin concentration 25 ppm causing the higher average efficiency transformation reach 17,50%.