Open AccessOptimization of extraction method for extracellular polymeric substances of Phanerochaete chrysosporium
Author(s) -
Jie Liu,
Wu Lei,
Ningjie Li,
Zhongwei Chen,
Qi Lan
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/371/4/042008
Subject(s) - phanerochaete , extracellular , extracellular polymeric substance , chrysosporium , centrifugation , polysaccharide , extraction (chemistry) , chromatography , chemistry , extracellular polysaccharide , polymer , biochemistry , biodegradation , biology , organic chemistry , enzyme , biofilm , bacteria , genetics
There are many methods for extracting extracellular polymeric substances of white rot fungi. In this experiment, we studied the efficiency of NaOH method, EDTA-2Na method and high-speed centrifugation method. Without destroying the cell structure and the activity of extracellular polymeric substances, we maximized the effect of extracellular polymeric substances. The results showed that in the ultrasonic centrifugation method, the polysaccharide concentration in the extracted EPS solution gradually increased with the increase of the ultrasonic action time. The concentration of polysaccharide in the EPS solution extracted under the ultrasonic action for 10 min and 12000 rpm was the highest (613.638 mg/L); on the whole, the ultrasonic treatment time has a greater impact on the extraction efficiency of polysaccharides in the extracellular polymer of Phanerochaete chrysosporium , and the polysaccharide concentration changes greatly. This study can provide useful information for the follow-up experiments related to extracellular polymeric substances of white rot fungi.