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Next-generation sequencing yields the complete mitochondrial genome of mud spiny lobster, Panulirus polyphagus (Crustacea: Decapoda) from Madura water
Author(s) -
Sapto Andriyono,
Md. Jobaidul Alam,
Heru Pramono,
Annur Ahadi Abdillah,
Hyunbeen Kim
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/348/1/012020
Subject(s) - biology , mitochondrial dna , spiny lobster , phylogenetic tree , ribosomal rna , transfer rna , gene , genome , genetics , evolutionary biology , zoology , crustacean , rna
The circular mitochondrial genome of the mud spiny lobster, Panulirus polyphagus was determined by next-generation sequencing (NGS) platform. The mitochondrial genome of P. polyphagus was 15,707 bp in length, which comprised 13 protein-coding genes, 22 tRNAs, two ribosomal RNAs (12S and 16S). A non-coding putative control region (739 bp) was located between 12S ribosomal RNA and tRNA- Ile . Except for COX1, 13 protein-coding genes initiated with the conventional start codon (ATG). The phylogenetic analysis with the mitogenomes in family Palinuridae showed P. polyphagus was clustered together with four congener species forming a clade, and Panulirus versicolor is closest to P. polyphagus.

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