Open AccessMicrobiological quality (pathogen E. coli bacteria) in the coastal environment of Dumai City, Riau Province
Author(s) -
Iesje Lukistyowati,
Feli Feliatra,
N. Nursyiwarni,
A. Qoriman,
T. Lekso,
Ahmad Mulyadi
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/348/1/012009
Subject(s) - escherichia coli , bacteria , microbiology and biotechnology , biology , chloramphenicol , antibiotic resistance , escherichia coli infection , microorganism , penicillin , veterinary medicine , antibiotics , medicine , biochemistry , gene , genetics
Escherichia coli is a water indicator microorganism. E. coli is found in almost water bodies such as lakes, rivers and seas that come from the feces of human and warm-blooded animals and waters contaminated with organic wastes. Microbiological quality is based on the presence of certain groups of microbes such as microbial pathogens (stomach disease), pollutants (especially E. coli) , toxin producers etc. This research was conducted in October 2018. The purpose of this study was to calculate the density of E. coli bacteria in the coastal city of Dumai. This study used the survey method, with 5 stations with 3 sampling points at each station. Calculation of the number of Escherichia coli colonies used the MPN (Most Probable Number) method. The density of E. coli bacteria ranged from 2.37 × 10 4 to 1.1 × 10 5 CFU / 100 ml. The highest number of Escherichia coli bacterial colonies was at stations 2 and 3, the lowest was at station 5. The results of the E. coli bacteria resistance test for seven isolates of E. coli and the results of Escherichia coli bacteria resistance to antibiotics showed that there were seven isolates of Chloramphenicol, which included resistance with inhibitory zones ranging from 2.17 to 10.70 mm, 9 bacterial isolates including intermediates with inhibitory zones ranging from 11.90 - 19.33 mm, and one isolate including sensitive with a zone of inhibition of 22.77 mm. In the Penicillin and Isoniazid antibiotics all resistant bacterial isolates with inhibition zones ranged from 0.80 to 4.70 mm and 1.50 - 4.67 mm respectively. The coastal waters of the city of Dumai have passed the threshold for cultivation.