Open AccessIdentification of suitable culture media for stimulation of Dendrobium ‘Earsakul’ growth
Author(s) -
Sasitorn Hualsawat,
K. Ngoenyu,
K. Phanya,
Sukanya Inthaisong,
S. Moranok,
S. Promaunand,
Piyada Alisha Tantasawat
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/346/1/012016
Subject(s) - dendrobium , micropropagation , slow growth , horticulture , plant growth , murashige and skoog medium , biology , botany , tissue culture , in vitro , biochemistry , macroeconomics , economics
Dendrobium is one of the most important economic cut-flowers in Thailand because of its attractive floral characteristics and popularity among consumers worldwide. However, its slow growth has prevented rapid mass micropropagation. The objective of this work was to stimulate Dendrobium growth in vitro using supplements, plant growth regulators and natural extracts. Dendrobium plantlets were cultured on VW0 media supplemented with 3 doses each of the energy drink Ready® (5, 10 and 15 mL/L), 6-benzylaminopurine (BAP; 1, 2 and 3 mg/L), V8® juice (100, 200 and 300 mL/L) and shrimp paste (2.5, 5 and 7.5 g/L) for 90 days. Plain VW0 media were used as control. The results showed that at 90 days the highest fresh weight (0.39 ± 0.05 g), plant height (3.99 ± 0.23 cm) as well as total root length (7.08 ± 1.30 cm) was obtained on VW0 media supplemented with 1 mg/L BAP. These were 1.8, 1.5 and 2.5-fold higher than those observed on plain VW0 media, respectively. However, the number of roots and number of leaves of plantlets cultured on this medium were not statistically significant. Results suggest that this culture medium is useful in promoting D. ‘Earsakul’ growth in vitro and may be applied for any future commercial micropropagation.