Open AccessHistopathological study of hepatopancreas and kidney of butini fish (Glossogobius matanensis) in Matano Lake, South Sulawesi, Indonesia, caused by metal contamination
Author(s) -
Nurul Sulfi Andini,
Hilal Anshary,
_ Wahyuni,
Adam Sukma Putra,
Dwi Kesuma Sari
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/343/1/012033
Subject(s) - hepatopancreas , histopathology , haematoxylin , kidney , biology , staining , eosin , fish <actinopterygii> , chemistry , pathology , fishery , endocrinology , biochemistry , medicine , genetics
Butini fish ( Glossogobius matanensis ) is one of the most endemic species of freshwater fish that lives in Matano Lake. The purpose of this study was to determine the histopathology of hepatopancreas and kidney from butini fish. The samples used were six Butini fishes with a size of approximately 18.5–20.5 cm. Organ preparations (hepatopancreas and kidneys) were fixed using 10% neutral buffered formalin (NBF), dehydrated using multilevel alcohol, embedded using paraffin, cut with a thickness of 5 μm, stained using Haematoxylin-Eosin (HE) then covered for microscopic observation. Data analysis used was qualitative descriptive. Measurement of metal content of Matano Lake water showed nickel content of <0.0184 mg/L, iron 0.0238 mg/L, zinc 0.0491 mg/L, and copper <0.0136 mg/L. Histopathological study on hepatopancreas showed fat degeneration and cell necrotic, while kidney showed renal tubular lysis, basal membrane detached, and cell necrotic. Thus, the high concentration of metal was thought to be related to the abnormal tissue of fish organs observed in this study.