Open AccessA study on the biological activity of biosynthesized nanoparticles of metal oxides
Author(s) -
А. М. Короткова,
Oksana B. Polivanova,
И. А. Гавриш,
M. Yu. Koroleva,
Е. Н. Баранова,
С. В. Лебедев
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/341/1/012176
Subject(s) - scanning electron microscope , nuclear chemistry , metal , morphology (biology) , nanoparticle , transmission electron microscopy , hexagonal crystal system , materials science , aqueous solution , nanomaterials , analytical chemistry (journal) , nanotechnology , chemistry , crystallography , metallurgy , chromatography , organic chemistry , composite material , biology , genetics
There was synthesized a number of nanopowders of metals oxides (Fe 2 O 3 , Co 3 O 4 , ZnO, and CeO 2 ) in the aqueous extract from Petroselinum crispum leaves. There was performed the physicochemical qualification of the synthesized nanomaterials by the methods of UV spectrophotometry and the scanning electron microscopy. SEM-visualization showed CeO 2 powders had a spherical morphology (from 14 nm to 500 nm), Fe 2 O 3 had a plate structure (more than 80 nm), and Co 3 O 4 was presented in small cubic particles with sharp edges (from 20 to 100 nm) stuck together in large units (~ 1 μm), ZnO was characterized by a spherical, oval and hexagonal structure (from 60 to 160 nm). The analysis of cytotoxicity of the biosynthesized powders was carried out as counting dead cells in the roots of T. vulgare stained with the vital dye Evans blue after 14 days of exposure with a preparation in a wide range of concentrations (from 10 −1 to 10 −5 M). The results showed a dose-dependent increase in cell death at a greater extent on the apex of the root compared to the basal area. A remarkable (over 50%) decrease in the cell viability was recorded after the treatment of plants with Fe 2 O 3 (more than 80%), 10 −1 M CeO 2 (84.7%), 10 −1 and 10 −2 M Co 3 O 4 (165 and 70%, respectively). However, a slight increase of viable cells was observed after the treatment with ZnO and CeO 2 in dilutions of 10 −4 to 10 −5 M (by 8% compared to the intact samples). In general, the cell viability of the seedling roots of T. vulgare after the exposure to the biosynthesized nanomaterials increased as follows: Fe 2 O 3 <Co 3 O 4 <CeO 2 <ZnO.