Open AccessDuckweed Quality Improvement Through Fermentation Using Trichoderma harzianum and Saccharomyces cerevisiae on Dry Matter, Ash and Crude Fat
Author(s) -
Hendi Setiyatwan,
Ellin Harlia,
Denny Rusmana
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/334/1/012030
Subject(s) - dry matter , fermentation , trichoderma harzianum , completely randomized design , nutrient , chemistry , food science , zoology , spore , horticulture , botany , biology , biological pest control , organic chemistry
Duckweed has been used as animal feed which contains high nutrients, ie proteins and amino acids, but the water content is high. This is a limitation in its use Therefore, research was conducted to improve dry matter, mineral content and lower duckweed crude fat content. Duckweed fermentation is divided into two stages, firstly, Trichoderma harzianum (Th) (3x10 7 spores/100 grams substrate) with added ZnCO 3 (186 ppm) and dl-methionine (286 ppm), secondly, Saccharomyces cerevisiae (Sc) (3x10 7 spores/100 grams of a substrate). Completed randomized design (CRD) was used in experimental design with 20 experimental units, the treatments consist of P1 (Fermentation using Th for 1day continued with Sc for 9 days), P2 (Th for 3 days continued with Sc for 7 days), P3 (Th 5 days continued with Sc 5 days), P4 (Th 7 days continued with Sc 3 days), P5 (Th 9 days continued with Sc 1 day) with four replications. Analysis of variance was conducted to know the treatment effect, then followed by the Duncan test to know the difference between treatments. Fermentation using Th and Sc has a significant effect (P<0.05) on dry matter, ash, and crude fat in the duckweed. The best duration of fermentation is Th for 3 days and Sc for 7 days (P2) that increasing dry matter (92.95%) and ash (21.35%), and lower crude fat (2.37%). Duckweed fermentation with the combination of Th for 3 days and continued Sc for 7 days with added dl-methionine and Zn yield the highest dry matter and ash content, and lowest crude fat contents.