Open AccessEnzymatic Hydrolysis Effect of Different Components of Bacillus licheniformis on Globinase
Author(s) -
Zurong Song,
Xuehua Feng,
Ali Tao,
Tao Chen
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/330/4/042060
Subject(s) - bacillus licheniformis , enzymatic hydrolysis , hydrolysis , ultrafiltration (renal) , chromatography , chemistry , enzyme , biochemistry , substrate (aquarium) , hemoglobin , biology , bacteria , bacillus subtilis , ecology , genetics
To replace the globin with blood globulin powder made from pig blood, to explore the enzymatic hydrolysis effect of different components of Bacillus licheniformis on globinase, and provide evidence for further development and utilization of pig blood. Orthogonal test was carried out by using different reaction temperature, substrate concentration, reaction time and pH value. The enzymatic hydrolysis conditions were optimized, and the optimum conditions were determined by enzymatic hydrolysis. The components of the strain were determined by one-way experiment. Through this experiment, it was found that the five stages after the separation and purification of the protease from Bacillus licheniformis passed through the ultrafiltration membrane, and the component of 50-100 nm has the best enzymatic hydrolysis effect with globin. The 50-100 nm segmentation of Bacillus licheniformis by ultrafiltration separation has the best globin enzymatic hydrolysis effect. It can be screened for deep processing of pig blood, which has great application prospects for pig blood processing and environmental protection in China.