Open AccessTriterpene alcohols and sterols of Siberian larch (Larix sibirica Ldb.) needles
Author(s) -
Darya S Mikson,
В. И. Рощин
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/316/1/012038
Subject(s) - chemistry , unsaponifiable , column chromatography , alkaline hydrolysis , organic chemistry , cycloartenol , chromatography , triterpene , petroleum ether , saponification , alcohol , potassium hydroxide , silica gel , ether , hydrolysis , fraction (chemistry) , sterol , extraction (chemistry) , biochemistry , medicine , alternative medicine , pathology , cholesterol
The composition of triterpene alcohols and sterols in the fraction of Siberian larch ( Larix sibirica Ldb.) conifer needles is considered in paper. The needles areextracted with propan-2-ol; resinous substances are extracted with petroleum ether (40–70°C);substances extracted with petroleum ether are divided into free acids and neutral substances. Neutral substances are separated in a silica gel column; eluent - petroleum ether supplemented with diethyl ether. The isolated esters fraction is hydrolyzed with potassium hydroxide in ethanol, and the “bound” acids are separated from unsaponifiable substances. Non-saponified substances are separated by chromatography into 5 fractions of phytosterols, the alcohol components of esters. The fractions are acetylated; the alcohol acetates are separatedonsilica gel with added silver nitrate. The isolated compounds was identified by NMR and GC-MS methods. Cycloartenol (6.82%, hereafter based onunsaponifiable substances), 24-methylenecycloartanol (6.75%), citrastadienol (7.69%), sitosterol (7.18%), cycloevcalenol (0.09%) are identified, obtussifoliol (0.01%), 24-methylenelophenol (0.10%), methyllophenol (1.84%) and campesterol (0.33%).