Open AccessThe modification of murashige and skoog media for efficient cultivation of gizella-5 and vsl-2 rootstocks in vitro
Author(s) -
Ibragim M. Bamatov,
Elina Minerovna Butsaeva,
Magomed M. Arsanov,
Dzhabrail M. Bamatov
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/315/4/042015
Subject(s) - rootstock , grafting , murashige and skoog medium , botany , nutrient , biology , horticulture , in vitro , chemistry , tissue culture , ecology , biochemistry , organic chemistry , polymer
This paper reports the effect of modified nutrient media on biotechnologically growing rootstock plants (Gizella – 5 and VSL – 2) at the stages of introduction and grafting. Murashige and Skoog medium was modified by adding 25 mg/l Chelate-Fe and 500 mg/l of Ca(NO) 3 to the basic nutrient at the stage of introduction of the plant. At the grafting stage 0.1 mg of 6-Benzylaminopurine (6-BAP) was added to Murashige and Skoog, where the effect of regeneration was 2 times higher than in experimental control. The results obtained prove that modified Murashige and Skoog medium is to be the best nutrient media for root-plant introduction and regeneration processes in Vitro followed by Chelate-Fe, Ca(NO) 3 and 6-BAP respectively.