Open AccessImmunohistochemical diagnostics of porcine reproductive and respiratory syndrome
Author(s) -
V.V. Stafford,
Anton G. Yuzhakov,
S.A. Raev,
Konstantin P. Alekseev,
Л. В. Костина,
Ya. B. Streltsova,
A. D. Zaberezhniy,
Т.И. Алипер
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/315/2/022044
Subject(s) - immunohistochemistry , porcine reproductive and respiratory syndrome virus , virus , pathology , tropism , arterivirus , lymph , monoclonal antibody , biology , respiratory system , lung , virology , parenchyma , antigen , antibody , medicine , immunology , disease , covid-19 , infectious disease (medical specialty) , anatomy
For the diagnostics of porcine reproductive and respiratory syndrome the method of immunohistochemical identification of the virus antigen inorgans and tissues was applied, which provides the reliability of the diagnosis of the disease and confirm the tropism of the virus to cells and tissues of pigs organs. The research was conducted based on own monoclonal antibodies to the virus nucleocapsid protein on experimentally infected pigs, and also on animals during the outbreaks of the disease in pig farms. The results of the immunohistochemical study during experimental infection of pigs with the PRRS virus indicate that the main pathological changes are localized in the lungs and bronchial lymph nodes, followed by accumulation of the virus in the alveolar macrophages and bronchial lymph nodes. The changes detected in other parenchymal organs are not associated with the accumulation of virus in them.