Open AccessEffect of α-Amylase and Glucoamylase Enzymes on Chemical Qualities of Vinegar from Banana Peel
Author(s) -
Juniawati,
widaningrum widaningrum,
Sri Usmiati,
Diny Agustini Sandrasari
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/309/1/012047
Subject(s) - banana peel , chemistry , food science , acetic acid , amylase , sugar , reducing sugar , alcohol , ethanol , fermentation , randomized block design , odor , enzyme , horticulture , biochemistry , organic chemistry , biology
The rate of banana consumption in Indonesia is reported to be increased. Abundant banana peels causes environmental problem. Peels of banana still have the potential to be utilized in the making of vinegar. Banana peels have high contents of fiber and carbohydrate, however breakage of polysaccharide bonds with enzyme is necessary. The α-amylase and glucoamylase are suitable enzymes to optimally break the polysaccharide bonds. The objective of this research was to determine the effect of α-amylase and glucoamylase on chemical qualities of banana-peel vinegar. The experimental design used was a Block Randomized Design with two factors and three replications. The ‘A’ factor was concentration of α-amylase at three levels (0.05%; 0.1%; 0.15%). The ‘B’ factor was concentration of glucoamylase at two levels (0.1%; 0.15%). Observation parameters included reducing-sugar, concentration of alcohol and acetic acid, and pH values. The high level of acetic acid was obtained from A2B1 (0.10% α-amylase; 0.10% glucoamylase) with an average value of 4.05% and pH of 3.52 on day-30 of fermentation period. An average value of reducing-sugar level on day-1 was 0.55% (g/ml) accompanied by alcohol content reduction of 0.386%. The best quality of banana-peel vinegar was resulted from the A2B1 treatment (0.10% α-amylase, 0.10% glucoamylase). Good quality vinegar was characterized by its aqueous liquid form, acetic acid odor with level at least 4%, residual alcohol at maximum level of 10%. The study has implications for the utilization of banana peel waste as natural preservatives.