Open AccessIsolation and preservation of microfungi from Indonesia islands
Author(s) -
Nurcahyani Wulandari
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/308/1/012079
Subject(s) - microfungi , isolation (microbiology) , agar , biology , botany , microbiology and biotechnology , bacteria , genetics
The research was aimed at utilizing more adequate methods of long-term preservation, cryopreservation (freezing at -80 °C). Samples obtained from three islands were used as sources of samples, i.e., Enggano, Riau, and Sumba. Direct isolation and single spore isolation were conducted to isolate the microfungi. All the cultures were grown on Potato Dextrose Agar (PDA). The growth rate of selected microfungi was calculated every day for a week. The growth rate for selected ten microfungi of each island, approximately 0.1-0.7 cm/day. All the cultures are maintained in InaCC (Indonesian Culture Collections), Cibinong, LIPI.