Open AccessEvaluation of the Environmental Factors Modulating Indole-3-acetic Acid (IAA) Production by Trichoderma harzianum InaCC F88
Author(s) -
Toga Pangihotan Napitupulu,
Atit Kanti,
I Made Sudiana
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/308/1/012060
Subject(s) - trichoderma harzianum , incubation , salinity , auxin , indole 3 acetic acid , tryptophan , axenic , fermentation , biomass (ecology) , chemistry , acetic acid , plant hormone , food science , horticulture , botany , biology , biochemistry , agronomy , amino acid , bacteria , biological pest control , gene , ecology , genetics
Indole-3-acetic acid (IAA) is one of the most common plant hormones that regulate many aspects of growth and development of plants. Trichoderma harzianum , a plant growth promoting fungus, has the ability to produce IAA. However, various environmental factors influence the formation of the hormone. The objective of this study was to optimize the environmental condition for the production of IAA by Trichoderma harzianum InaCC F88 strain. The effects of L-tryptophan concentration, temperature, pH, salinity, and incubation time on IAA production and biomass were studied. The in vitro evaluations were carried out in the axenic condition of Luria-Bertani Medium. Supplementation of 1% L-tryptophan in the medium gives maximum IAA production. The most favourable initial pH and temperature for IAA production are 6.0 and 27°C, respectively. In the salinity test, the medium containing 1% NaCl yielded maximum IAA formation. After 4 days of incubation, the concentration of IAA reached equilibrium. Hence, optimum IAA production in liquid fermentation could be achieved by manipulating those factors.