Open AccessCallus induction and in vitro mass culture of adventitious roots from leaf segment explants of Dendropanax morbifera Lev.
Author(s) -
Johan Sukweenadhi,
JungHye Choi,
Yujin Kim,
Lalitha Kaliraj,
Suleman Abid,
Jung Yong Ahn,
DeokChun Yang
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/293/1/012024
Subject(s) - callus , explant culture , sucrose , botany , murashige and skoog medium , auxin , biology , in vitro , tissue culture , horticulture , food science , biochemistry , gene
Dendropanax morbifera Lev. is a unique species and natively found in Korea and distributed in the South regions, such as Jeju, Goheung, and Wando. In this study, tissue culture system for native D. morbifera was developed. The callus from native D. morbifera leaves was cultured on Woody Plant Media (WPM) supplemented with 30 g L −1 of sucrose, with addition of 2.4-D and bA [(0.5, 1.0, 2.0) mg L −1 ], separately and mixed. After 5 wk of culture, the highest induction of callus was obtained from 0.5 mg L −1 of 2.4-D mixed with 2.0 mg L 1 of BA. Adventitious root formation on different media (MS, WPM, B5) with various auxins (IBA, IAA, and NAA) and different concentration [(0, 1, 3, 5) mg L −1 ] were tested. After 8 wk of culture, WPM showed better induction of adventitious root. The highest induction of adventitious root was obtained on 3.0 mg L −1 IBA. Root growth was best in WPM liquid medium with 3.0 mg L −1 of IBA and 30 g L −1 of sucrose. The same formulation with modified ½ WPM was successfully established in vitro adventitious roots culture in 18-L bioreactor system. This study also proposed as the mass production technique of adventitious roots from native D. morbifera .