Open AccessThermal and Reused Stability of Immobilized Lipase in Carrageenan
Author(s) -
Fandhi Adi Wardoyo,
Fitria Fatichatul Hidayah
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/292/1/012028
Subject(s) - lipase , hydrolysis , catalysis , chemistry , carrageenan , thermal stability , immobilized enzyme , chromatography , enzyme , organic chemistry , biochemistry
Lipase is one kind of enzyme that is widely used as a catalyst in industrial and medical. Lipase has a great catalytic ability, but is easily affected by temperature, and is also difficult to separate at the end of the reaction. This causes most of lipase used only in one reaction cycle. Therefore an enzyme immobilization is needed, so that lipase can maintenance it’s activity in high temperatures, and can also be separated at the end of the reaction so that it can be re-used. This study aimed to determine the thermal stability and re-used stability of the immobilized lipase in carrageenan. The method used for immobilizing lipase is through entrapment method. The effectiveness of immobilized lipase tested through the hydrolysis reaction of palm oil. The result was found that the immobilized lipase in carrageenan was able to maintain its catalytic activity up to 50 °C and also up to five reaction cycles.