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Preliminary study of biodegradability of starch-based bioplastics using ASTM G21-70, dip-hanging, and Soil Burial Test methods
Author(s) -
Rossy Choerun Nissa,
Anti Khoerul Fikriyyah,
Azween Abdullah,
Sri Pudjiraharti
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/277/1/012007
Subject(s) - bioplastic , biodegradation , starch , biodegradable plastic , materials science , glycerol , pulp and paper industry , aspergillus niger , mixing (physics) , composite material , food science , chemistry , waste management , engineering , organic chemistry , physics , quantum mechanics
Biodegradability testing methods being used nowadays have many disadvantages; they are time-consuming, inefficient medium used, and too much sample needed to do the test. This work aimed to study the biodegradability of starch-based bioplastics by modified ASTM G21-70 method using Salt Agar (SA) medium, dip-hanging method using sterile water, and Soil Burial Test (SBT) method. Bioplastics were prepared by mixing cassava starch and glycerol with a ratio of 3:1 (%, w/w) through a series of processes: (1) blending of starch and glycerol for 3 min, (2) extruding of the starch-glycerol mixture by using a single screw extruder at 80-130°C, and (3) compression molding at temperature and pressure of 150°C and 50 kgf/cm 2 , respectively. Aspergillus niger was used as bioplastic-degrading fungi for the modified ASTM G21-70 and dip-hanging methods, while compost-soil was used as a source of bioplastic-degrading microbes in SBT method. Bioplastics of 2x2 cm in size were applied to the tests for 10 days. The growth of fungi on the surface of bioplastics was observed visually at two days intervals. A. niger grew well on the surface of bioplastic sample in modified ASTM G21-70 method, indicated that the bioplastic could be degraded by the fungi. On the other hand, the growth of A. niger was poor in the dip-hanging method, even though weight loss of 11.5% occurred. Physical properties changing were indicated in the SBT method. On the 10 th day, cracks were observed on the surface of the bioplastic sample, the color of the sample became darker even the bioplastic became fragile, and the weight loss reached 29.89%.

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