Open AccessThe Quality of Ettawa Crossbreed Sperm: The Treatment with a Combination of Cryoprotectant in Tris Diluents
Author(s) -
Dwi Cahya Prastya Rini,
Siti Zaenab,
Samsun Hadi,
Fuad Jaya Miharja
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/276/1/012047
Subject(s) - cryoprotectant , glycerol , sperm , cryopreservation , diluent , andrology , sperm motility , yolk , tris , semen , semen cryopreservation , sperm quality , chemistry , biology , food science , biochemistry , medicine , nuclear chemistry , embryo , fishery
The formation of ice crystals easily damages the sperm of goats during the freezing process. This study aimed to determine the optimum treatment of cryoprotectant DMSO, glycerol, and a combination of DMSO with glycerol in Tris-egg yolk diluents to maintain the quality of frozen sperm of ettawa breeds. This study used four male goats of which semen was collected twice a week using an artificial vagina. The independent variables in this study were 6% DMSO, 6% glycerol, and a combination of 3% DMSO: 3% glycerol, negative control (without treatment), and positive control (7% glycerol). The sperm quality parameters include motility, viability, and abnormalities. Data collection techniques used observation techniques before and after freezing. The data were tested statistically using one-way ANOVA and Duncan test. The results showed that the combination treatment of 3% DMSO: 3% glycerol produced significant motility, viability, and abnormalities (P <0.05) compared to other treatments. These results indicated that the addition of a combination of DMSO and glycerol in egg yolk thinners successfully protected sperm from various stresses during the cryopreservation process to maintain the sperm quality.