Open AccessStudy on HPLC Fingerprint of Clematis manshurica Rupr from Different Areas
Author(s) -
Juan Kong,
Xulong Huang,
Xiaosong Yang,
Lingling Zhang,
Xiaofen Li,
Qian Zeng,
Hongmei Wu,
Xiangpei Wang
Publication year - 2019
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/267/6/062053
Subject(s) - clematis , high performance liquid chromatography , phosphoric acid , fingerprint (computing) , chromatography , chemistry , chromatographic separation , similarity (geometry) , traditional medicine , computer science , organic chemistry , artificial intelligence , medicine , image (mathematics) , herb , medicinal herbs
Objective: To establish HPLC fingerprint of Clematis manshurica Rupr from different areas. Method: 10 batches of Clematis manshurica Rupr was analyzed by RP-HPLC that the methods were used Odyssil-C 18 chromatographic column, with the mobile phase of acetonitril-0.1% phosphoric acid solution, the flow rate was 0.8mL/min, the wavelength was 210 nm, and column temperature was 30°C. And the similarity was evaluated by similarity evaluation software. Result: The HPLC-UV fingerprint of Clematis manshurica Rupr was established, and 20 common peaks were determined, the similarity was between 0.974-0.823, indicating that the differences of Clematis manshurica Rupr from different regions were significant. Conclusion: The analytical method has good separation degree and feasibility, and it can provide a reliable basis for the quality control of Clematis manshurica Rupr.