Open AccessTranscriptome-targeted analysis of human peripheral blood-derived macrophages when cultured on biomaterial meshes
Author(s) -
Camilo Mora-Navarro,
Emily W Ozpinar,
Daphne Sze,
David P. Martin,
Donald O. Freytes
Publication year - 2021
Publication title -
biomedical materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 72
eISSN - 1748-605X
pISSN - 1748-6041
DOI - 10.1088/1748-605x/abdbdb
Subject(s) - transcriptome , in vivo , inflammation , in vitro , biomaterial , wound healing , microbiology and biotechnology , immune system , biomedical engineering , materials science , medicine , immunology , gene expression , biology , gene , biochemistry
Surgical meshes are commonly used to repair defects and support soft tissues. Macrophages (M φ s) are critical cells in the wound healing process and are involved in the host response upon foreign biomaterials. There are various commercially available permanent and absorbable meshes used by surgeons for surgical interventions. Polypropylene (PP) meshes represent a permanent biomaterial that can elicit both inflammatory and anti-inflammatory responses. In contrast, poly-4-hydroxybutyrate (P4HB) based meshes are absorbable and linked to positive clinical outcomes but have a poorly characterized immune response. This study evaluated the in vitro targeted transcriptomic response of human M φ s seeded for 48 h on PP and P4HB surgical meshes. The in vitro measured response from human M φ s cultured on P4HB exhibited inflammatory and anti-inflammatory gene expression profiles typically associated with wound healing, which aligns with in vivo animal studies from literature. The work herein provides in vitro evidence for the early transcriptomic targeted signature of human M φ s upon two commonly used surgical meshes. The findings suggest a transition from an inflammatory to a non-inflammatory phenotype by P4HB as well as an upregulation of genes annotated under the pathogen response pathway.