Open AccessChemical characterization of extracellular vesicles of mesenchymal stromal cells: TOF-SIMS and BCARS approach
Author(s) -
Arseny Aybush,
А. А. Гулин,
A A Kuzoiatova,
Margarita Gubina,
Ф. Е. Гостев,
М. С. Сырчина,
А. Н. Ермаков,
Е. А. Супруненко,
В. А. Надточенко
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/2086/1/012107
Subject(s) - mesenchymal stem cell , extracellular vesicles , microvesicles , paracrine signalling , stromal cell , microbiology and biotechnology , vesicle , chemistry , extracellular , nucleic acid , exosome , biochemistry , biology , biophysics , membrane , microrna , cancer research , receptor , gene
Paracrine functions of mesenchymal stem (stromal) cells (MSCs) rely, at least partly, on membrane-bound extracellular vesicles (EVs) with rich composition of lipids, nucleic acids and signaling proteins. Elucidation the underlying chemistry could potentially lead to MSCs-free therapy. However, the secretome of MSCs (EVs’ composition) is non-static and depends on many other factors including surrounding cells and medium. Thus, the research techniques must be able to provide not only bulk but microscopy-scale data within a reasonable time frame. Two of these label-free techniques are subject of this work toward the question of chemical composition of the EVs.