Open AccessDesign and application of a fluorescent probe for rapid detection of H2S
Author(s) -
Sa Su
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/2009/1/012077
Subject(s) - hydrogen sulfide , fluorescence , endogeny , reactive oxygen species , chemistry , antioxidant , biophysics , in vitro , selectivity , biochemistry , sulfide , azide , biology , organic chemistry , sulfur , physics , quantum mechanics , catalysis
H 2 S is an endogenous gaseous signal molecule with cell protective function, which plays a critical part in many physiological and pathological processes. On the one hand, abnormal fluctuations of the concentration of hydrogen sulfide in the human body are closely related to diseases such as Down's syndrome, Alzheimer's, diabetes and cirrhosis of the liver. On the other hand, acts as an antioxidant in the body, hydrogen sulfide can protect cells from damage caused by reactive oxygen species. Therefore, it is indispensible to detect hydrogen sulfide in living organisms. A fluorescent probe—NX-H 2 S, was constructed using azide group as the recognition group and naphthalimide as the framework. The spectroscopic properties of the probe were explored comprehensively. The experiment results show that NX-H 2 S exhibits not only a fast response (5 min) but also excellent sensitivity and selectivity toward H 2 S. In addition to the rapid detection of H 2 S in vitro, NX-H 2 S can also be successfully applied to the fluorescence imaging analysis of endogenous and exogenous H 2 S in living cells.