Open AccessGlypican-3 electrochemical aptasensor based on CuO-rGO nanocomposite
Author(s) -
Huafu Feng,
Mingming Bao,
Mei Lian,
Guiyin Li
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/2009/1/012071
Subject(s) - graphene , differential pulse voltammetry , nanocomposite , materials science , aptamer , detection limit , oxide , electrochemistry , chemical engineering , linear range , cyclic voltammetry , hydrogen peroxide , electrode , nanotechnology , nuclear chemistry , chemistry , chromatography , organic chemistry , biology , engineering , metallurgy , genetics
Herein, a novel electrochemical immunosensor was fabricated based on copper oxide-reduction graphene oxide (CuO-rGO) nanocomposites for Glypican-3 (GPC3) detection. Firstly, CuO-rGO nanocomposite as nanoenzyme with good peroxidase-like properties is synthesized by One-step hydrothermal. Then CuO-rGO nanoenzyme captures GPC3 aptamer to form a signal probe, finally GPC3 antibody is used as the recognition probe to form an "antibody-antigen-aptamer" sandwich structure. Due to the peroxidase-like properties of the probe, this sandwich structure can effectively catalyze the reaction between hydrogen peroxide (H 2 O 2 ) and silver nitrate (AgNO 3 ) and AgNPs is deposited on the surface of the electrode to amplify the final measured current. The Differential Pulse Voltammetry (DPV) method was employed to record the response signal of the immunosensor. The current response presented a linear relationship to the GPC3 concentration in the range of 1 to 40 μg/mL with the detection limit of 0.45 μg/mL.