Open AccessMALDI MS/MS study of As(III) complexes with glutathione, glutathione disulphide and other ligands
Author(s) -
Anpoli
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1960/1/012007
Subject(s) - chemistry , glutathione , redox , intramolecular force , thiol , denticity , ligand (biochemistry) , crystallography , stereochemistry , inorganic chemistry , biochemistry , crystal structure , receptor , enzyme
The formation of complexes between As(III) and two redox couples GSH/GSSG, Cys/Cyst, and Cyt c , was evaluated by MALDI MS and MS/MS. Structural elucidation of complexes is reported, and the redox properties are discussed. Particular attention was addressed to the gas-phase ion chemistry of high molecular weight species (i.e., [As(GSH) 4 ] + ). The formation of the oxidized ligand from complexes where a thiol free group is present, gives evidence on an intramolecular redox process initiated by a laser induced oxidation step. MS/MS experiments also demonstrated that GSSG, Cyst and Cyt c act as bidentate ligands, determining the formation of cyclic structures.