Open AccessDetermination of Motility and Charge Type of Mencit Sperm (Mus musculus) Through Study of Exposure Electrical Fields
Author(s) -
E. Trimayanti,
Siti Nurul Khotimah,
Indra Wibowo,
Sparısoma Viridi
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1949/1/012021
Subject(s) - sperm , hemocytometer , electric field , andrology , sperm motility , motility , biology , microbiology and biotechnology , physics , medicine , biochemistry , quantum mechanics
Analysis of sperm quality is still one of the most important tools for evaluating a man’s fertility. Sperm quality includes several aspects including motility and viability. Mice ( Mus musculus ) are usually used as experimental animals because the reproductive system is almost the same as human. The purpose of this study is to determine the motility, viability, and type of charge of sperm mice ( Mus muculus ) through the study of exposure electric fields in an ex vivo condition. Observations were made in the time At for sperm without influence and influenced by an electric field. Mice sperm is diluted with a solution of Phosphate Buffered Saline (PBS). The liquid is inserted into the hemocytometer and then placed between two iron plates connected with a DC voltage source. The distance between the two plates d = 7.0 cm. The magnitude of the voltage used is Δv = 0 (control) and Δv = 12.5 V. Sperm movement was observed using a fluorescence microscope then recorded using NIS Elements software. Then the movement of sperm is analyzed using Tracker software. Sperm without an electric field (Δv = 0) has a viability of 60 minutes longer than sperm affected by an electric field (ΔV = 12.5 V) which has a viability of 30 minutes. The sperms move almost randomly in all directions without the influence of the electric field but tend to move to a positive plate when under the influence of the electric field. Based on the direction of the movement of mice sperm, the sperm is thought to be negatively charged. From the results of this study, it was found that the fast zig-zag motion had a velocity v > 37 μm/s.