Open AccessAntimicrobial activity of secondary metabolites of endophytic bacteria F4 of papaya leaf (Carica papaya L)
Author(s) -
Purbowatiningrum Ria Sarjono,
Devi Silvia,
Nies Suci Mulyani,
Ismiyarto Ismiyarto,
Ngadiwiyagadiwiyana,
Nor Basid Adiwibawa Prasetya,
Yosie Andriani
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1943/1/012172
Subject(s) - antimicrobial , bacteria , phytochemical , secondary metabolite , carica , biology , aspergillus niger , minimum inhibitory concentration , escherichia coli , microbiology and biotechnology , gram negative bacteria , gram positive bacteria , metabolite , food science , chemistry , botany , biochemistry , genetics , gene
Papaya leaves have been known to contain secondary metabolite compounds as antimicrobial, so that endophytic bacteria is also suspected of having secondary metabolite compounds as antimicrobial. In previous research, endophytic bacteria have been isolated from papaya leaf and obtained 5 endophytic bacteria which were F1, F2, F3, F4 and F5. Three bacterias that have been known for their antibacterial activity were F1, F3, and F5. This research aims to determine the antimicrobial activity of F4 endophytic bacteria. The results showed that single isolates of endophytic bacteria F4 is a type of Gram-positive bacteria with a basil cell shape (stem). F4 endophytic bacteria has 3 growth phases; they are logarithmic phase occurs at 0-8 hours, stationary phase at 8-34 hours, and phase of death at 34-46 hours. Phytochemical results showed that MS18 contained saponin group compounds, while MS36 contained alkaloids and saponin group compounds. The highest antimicrobial activity was in the death phase, MS36 with minimum inhibitory concentration (KHM) to Escherichia coli , Candida albicans , and Bacillus subtillis were 15.5 mg/mL, respectively; 16.5 mg/mL and 16.5 mg/mL by disc diffusion method, whereas to Aspergillus niger of 0.5 mg/mL by dry weight method.