Open AccessCharacterization of green silver nanoparticles of Graptophyllum pictum leaf extract: from the localized surface plasmon resonance to the antimicrobial activity
Author(s) -
Synodalia C. Wattimena,
D. R. Silooy,
Philipus J. Patty
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1943/1/012065
Subject(s) - surface plasmon resonance , nanoparticle , fourier transform infrared spectroscopy , silver nanoparticle , antimicrobial , particle size , particle (ecology) , staphylococcus aureus , spectroscopy , materials science , resonance (particle physics) , antibacterial activity , analytical chemistry (journal) , chemistry , nuclear chemistry , nanotechnology , chemical engineering , chromatography , organic chemistry , bacteria , biology , ecology , genetics , physics , particle physics , quantum mechanics , engineering
This study aims to synthesize silver nanoparticles using leaf extract of Graptophyllum pictum and to characterize their properties, starting from localized surface plasmon resonance, functional groups and the particle size distribution, to their antibacterial activity. The measurement of the wavelength of localized surface plasmon resonance was conducted using UV-VIS spectroscopy, while FTIR spectroscopy was used to identify the chemical bonds of organic compounds in the particle. The particle size distribution was analyzed using TEM. The spectrophotometric method was used to assess the antimicrobial properties of the particles against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli . For this purpose, OD-620 of the bacterial sample was compared to OD-620 of the bacterial sample mixed with the silver nanoparticles, where the data was taken in 24 hours. The wavelength of localized surface plasmon resonance was found to be 455 nm, while FTIR spectrum showed the chemical bonds of organic compounds, denoting the presence of the extract on the particle. The particles were mostly spherical with diameters varying from 5.4 nm to 50.6 nm and the mean diameter was found to be 21.5±9.9 nm. The results from the antimicrobial assessment show that Graptophyllum pictum silver nanoparticles inhibit the growth of both S. aureus and E. coli , where during 24 hours of observation time, the particles affected E. coli , faster than the particles affected S. aureus .