Open AccessEffect of Rhodamine b Against The Number of Primary Follicles in White Rats (Rattus norvegicus)
Author(s) -
Huda Rohmawati,
Meirna Eka Fitriasnani,
Weni Tri Purnani,
Rahma Kusuma Dewi
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1899/1/012070
Subject(s) - rhodamine b , rhodamine , group b , andrology , positive control , white (mutation) , analysis of variance , staining , biology , ovary , medicine , endocrinology , traditional medicine , biochemistry , genetics , fluorescence , physics , photocatalysis , quantum mechanics , gene , catalysis
Rhodamine b is misused as a food coloring. Excessive use is certainly harmful to health. The purpose of this study proves that Rhodamine b can reduce the number of primary follicles in the ovaries of white rats. The design of this study is true experimental with Post Test only control group design. Sample in the research used were female white rats (Rattus norvegicus) aged 2-3 months with a bodyweight of 150-250 grams in the amount of 10 animals which were divided into 2 groups: negative control group, positive control group. Rhodamine b is given for 36 days at a dose of 18 mg / 200 gr orally. The number of primary follicles is calculated by HE staining. Data were analyzed and conducted One Way Anova test. The results showed that the administration of Rhodamine b significantly reduced the number of primary follicles in the ovaries of white rats. This is because rhodamine b is capable of binding with halogen compounds which are reactive so that it can damage cells. Based on the results of the analysis showed that Rhodamine b can reduce the number of primary follicles in the ovaries of the white rat.