Open AccessIdentification of sea urchin gonads chemical compounds using thin-layer chromatography from Bokory island, Southeast Sulawesi
Author(s) -
Karmilah,
Musdalipah,
Nur Saadah Daud,
Reymon,
Yulianti Fauziah
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1899/1/012050
Subject(s) - sea urchin , phytochemical , paracentrotus lividus , thin layer chromatography , gonad , biology , traditional medicine , botany , chemistry , chromatography , ecology , anatomy , medicine
Southeast Sulawesi is one area that has a diversity of potential marine biota as medicinal ingredients, including the sea urchin gonads. Empirically coastal communities have been hereditary, consuming sea urchin gonads as a source of high protein. Considering its potential as a raw material for medicines, it is necessary to conduct a series of tests to guarantee its safety and efficacy. This study aims to determine the chemical compounds of sea urchin gonad extract ( Diadema setosum L) by Thin-Layer Chromatography (TLC). The research method uses a Pre-experimental design with a one-shot case study model. Identification of metabolites of sea urchin gonad extracts was carried out in two stages: phytochemical screening and identification by TLC method by UV-Vis. Samples were dried by the Freeze-drying method to get 335.11 g of dry samples and 58.06 g of extracts. The results of the test of the content of water-soluble and ethanol-soluble sea urchin extract were 5.73% and 3.72%. The results showed that in phytochemical screening, ethyl acetate extracts of the positive marine gonads contained alkaloids, saponins, and steroids. In addition, UV-vis 254 nm and 366 nm TLC methods showed alkaloids and saponins. The Rf values are 0.4 and 0.68, respectively.