Open AccessIsolation and Identification of Ethyl Acetate Extract Secondary Metabolite Compound of Kayu Jawa Bark (L. Coromandelica)
Author(s) -
Sudding,
Pince Salempa,
Nurhikmah
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1899/1/012035
Subject(s) - bark (sound) , reagent , chemistry , ethyl acetate , fractionation , chromatography , metabolite , maceration (sewage) , secondary metabolite , acetic acid , nuclear chemistry , organic chemistry , biology , materials science , biochemistry , ecology , gene , composite material
This study aims to isolate the secondary metabolite compounds contained in the ethyl acetate extract in bark of L.coromandelica. Isolation was carried out through several stages, ranging from maceration, fractionation, purity testing, and identification. The isolates obtained was white crystals in the form of needles with a melting point 133-134°C, with Lieberman-Buchard reagents gave positive results. The IR spectrum absorption area showed the existence of several functional groups at wavelength namely 3446.79 cm −1 (O-H), 2935.66 cm −1 (C-H); 2866.22 cm −1 (C-H), 1463.97 cm −1 (-C-H2), 1377.17 cm −1 (-C-H3), 1643.35 cm −1 (C=C), 1047.35 cm −1 (C-O). Based on the reagent test and IR spectrum data, the crystals obtained are a group of steroid compounds.