Open AccessEnhancing tools in lateral flow assay for improving detection limit and working range
Author(s) -
Nadezhda A. Taranova,
Anatoly V. Zherdev,
Boris B. Dzantiev
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1889/5/052042
Subject(s) - detection limit , limit (mathematics) , range (aeronautics) , colloidal gold , materials science , chromatography , nanotechnology , nanoparticle , analytical chemistry (journal) , chemistry , biomedical engineering , computer science , mathematics , medicine , composite material , mathematical analysis
This work is directed to the estimation of the analytical parameters for improved immunochromatographic tests. A combination of 2 approaches has been proposed to decrease the detection limit of troponin T, a biomarker of acute myocardial infarction. The first approach consists of replacing spherical gold nanoparticles, which are common markers in immunochromatography, with alternative markers, such as gold nanoflowers (AuNFs). The second approach consists of the formation of large aggregates in the analytical zone from AuNFs due to the biotin–streptavidininteraction. The improved tests demonstrated a 10-fold lowering of the detection limit (1.2 ng/ml instead of 11.1 ng/ml) and a 3-fold expanding of the working range of the determined concentrations.