Open AccessPurification and Determination of The Arginase Enzyme Activity from Kidney Patients
Author(s) -
Nuha Ali Hadi Al-Samarrai,
Nadia Ahmed Salih
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1879/2/022057
Subject(s) - arginase , sephadex , creatinine , uric acid , urea , albumin , chromatography , enzyme , globulin , chemistry , medicine , enzyme assay , urea nitrogen , endocrinology , biochemistry , amino acid , arginine
The current study measuring the effectiveness and purification of the enzyme (arginase) in the serum of kidney diseases samples were collected from Samarra General Hospital from October to December 2019, 60 samples were collected from the infected and 25 samples from he healthy people control group of ages 30-55 years, the effectiveness of the control group was 6.87 ± 2.95, and the patient group was 25.71 ± 8.39., the purification process was carried out using Sephadex G-200 and EDEA cellulose - using a column of separation with dimensions (32 x 1.5nm), the molecular weight of the enzyme was 245 kDa. The study included measuring the level of urea 83.7 ± 23.4mg/dl, Creatinine 2.85 ± 1.19 mg/dl, Total protein 3.8 ± 0.6 mg/dl, Albumin 4.120 ± 0.02 mg/dl, Uric acid 5.19 ± 1.52 mg/dl and Globulin was 1.29 ± 0.01 mg/dl. at P≤0.01.