Open AccessProduction of Cellulase and bioethanol by ethanol-tolerant coculture of Bacillus cereus and Fusarium solani
Author(s) -
Mohammed Salah Abood,
Haider Hamzah,
Dhafer Fakri Al-Rawii
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1879/2/022016
Subject(s) - cellulase , fermentation , food science , fusarium solani , chemistry , reducing sugar , cellulose , ethanol , cynodon dactylon , ethanol fuel , sugar , bacillus cereus , biofuel , hydrolysis , botany , bacteria , biochemistry , biology , microbiology and biotechnology , genetics
Cellulase is an enzyme produced by fungi and bacteria that hydrolyzes cellulose by breaking down the glycosidic bond, β-1,4 that binds sugar glucose units. Bioethanol and cellulase enzyme were produced by ethanol-tolerant of Bacillus cereus co-culture. So the production of filamentous solani were using the wastes of Cynodon dactylon L. The highest activity of enzyme was at 96 hour/30°C of incubation. The optimal pH value was 7.5, while the carbon concentration was 3%. On the other hand, the best inoculum ratio was 0.5/100 mL (52 × 10 8 cell/mL bacterial cells and 10 5 conidia/mL of the fungus). In order to test the efficiency of ethanol production by the co-culture by using different concentrations of pure ethanol was measured in the solid medium. Both Bacillus sp. and F. solani tolerated incubation with 5% ethanol. The best treatment when using 0.5% sulfuric acid which gave the best concentration of reduced sugars and the results showed a difference in the concentration of sugars produced by the fermentation process, it was 3.9 mg/mL in the first day, whereas it was 2.51 mg/mL after 120 hours of fermentation, the concentration of bioethanol produced after fermentation was 195 g/L. In conclusion Cynodon dactylon L. wastes were treated by sulfuric acid, cellulase digestion, and autoclave treatment to be a good source of reducing sugars.