Open AccessThe effect of dimethyl sulfoxide on Corynebacterium pseudotuberculosis biofilm: An in silico prediction and experimental validation
Author(s) -
Mohamad Fakhri Yaacob,
Faez Firdaus Jesse Abdullah,
Norashirene Mohamad Jamil,
Norfatimah Mohamed Yunus,
Shafiq Aazmi,
Mohd Fakharul Zaman Raja Yahya
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1874/1/012055
Subject(s) - corynebacterium pseudotuberculosis , caseous lymphadenitis , dimethyl sulfoxide , in silico , biofilm , microbiology and biotechnology , biology , pathogen , chemistry , bacteria , biochemistry , gene , genetics , organic chemistry
Corynebacterium pseudotuberculosis is a Gram-positive pathogen that commonly causes caseous lymphadenitis which occurs in sheep, goats, cattle, buffalo and horses. This disease has long been shown to be a major cause of economic loss on sheep industries. Dimethyl sulfoxide (DMSO) is known to be effective against a wide spectrum of pathogens however, its efficacy against C. pseudotuberculosis biofilm remains uncertain. The objective of this study was to predict the antibiofilm potential of DMSO against C. pseudotuberculosis using in silico protein interaction network analysis and experimentally determine the antibiofilm activity using standard microplate assay system. As compared to the protein interaction network of S. typhimurium biofilm that had previously been shown to be inhibited by DMSO, the protein interaction network of C. pseudotuberculosis showed similar nodes, hub proteins and functional linkages between glycolytic enzymes. Further experimental validation revealed that the treatment with DMSO significantly (p<0.05) inhibited C. pseudotuberculosis biofilm at all tested concentrations (1.56% - 50%). The findings from the present study suggest the potential application of DMSO in controlling caseous lymphadenitis in ruminants.