Open AccessTyrosinase inhibitory activity of Garcinia daedalanthera Pierre
Author(s) -
Neneng Siti Silfi Ambarwati,
Berna Elya,
Yesi Desmiaty,
Islamudin Ahmad
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1869/1/012019
Subject(s) - tyrosinase , kojic acid , chemistry , ethyl acetate , skin whitening , hydroxylation , enzyme , melanin , maceration (sewage) , biochemistry , chromatography , pharmacology , biology , materials science , composite material , active ingredient
The tyrosinase enzyme catalyzes the first stage in two reactions in the synthesis of melanin (the hydroxylation of l-tyrosinase becomes 3,4-dihydroxyphenylalanine (l- DOPA) and oxidation from L-dopa to dopaquinone). Hyperpigmentation in human skin is a general phenomenon that is not desirable. Researchers were encouraged to identify potential new tyrosinase inhibitors for cosmetics, especially anti-hyperpigmentation. This study purposed to determine the inhibitory activity of the tyrosinase enzyme from Garcinia daedalanthera Pierre leaves. The extract was obtained by maceration successively method. The anti-tyrosinase assay used the spectrophotometric method at 490 nm. The enzyme used tyrosinase from mushrooms lyophilized powder (Sigma), and the substrate used 3,4-dihydroxy-L-phenylalanine, L-DOPA (Sigma). The tyrosinase inhibitory assay results of 100 ppm (triplicate) showed the leaves extract, including ethyl acetate extract of 33.42 ± 5.98 %, hexane extract was 50.67 ± 0.47 %, and methanol extract of 50.68 ± 1.87 %, respectively. Moreover, the stem bark has activity as follows methanol extract of 43.76 ± 1.41 % and the ethyl acetate extract of 55.71 ± 2.80 %. Percentage inhibition of the positive control (kojic acid) was 65.07 ± 0.03 % at 100 ppm.