Open AccessExtraction of bixin from annatto seeds with microwave
Author(s) -
E. Erdawati,
E. Allanas,
W. Wesnina
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1869/1/012016
Subject(s) - extraction (chemistry) , yield (engineering) , pigment , solvent , distilled water , ethyl acetate , chromatography , high performance liquid chromatography , chemistry , carboxylate , ultraviolet visible spectroscopy , spectroscopy , infrared spectroscopy , nuclear chemistry , organic chemistry , materials science , physics , quantum mechanics , metallurgy
This study aims to determine the effect of extraction time (5,10,15 minutes) and the ratio of the aquadest solvent: ethyl acetate (1: 6.5, 1: 7, 1: 8 v/v) to the yield of pigment. The optimum condition was found in the ratio of the solvent of distilled water: ethyl acetate (1: 7 v/v) extraction time of 15 minutes with low microwave power resulted in maximum pigment yield of 0.469%. The isolate compounds were analyzed using IR spectroscopy, mass spectroscopy, and High-Performance Liquid Chromatography (HPLC) showed that the isolates were boxing pigments having a molecular weight of 394 g/Mol with wavenumbers for OH groups, C = O carboxylate groups, CH bonds, duplicate C = C, and CO ester bonds. As well as showing the retention time of standard and standard pigments at minutes to 12.849 and 12.843.