Open AccessAlternative technique for direct immobilization of biomolecules
Author(s) -
E. Belina,
J. Karadjov,
K Shindarova,
Elitsa Pavlova,
T L Dimitrowa,
Hristo Kisov,
Georgi Dyankov
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1859/1/012061
Subject(s) - biosensor , biomolecule , surface plasmon resonance , matrix (chemical analysis) , transducer , nanotechnology , embedding , materials science , chemistry , computer science , chromatography , nanoparticle , physics , quantum mechanics , artificial intelligence
The conventional approach to immobilization of biomolecules on the transducer of biosensors requires their embedding into the so-called embedding matrix. The procedure is complicated and laborious and performed by chemical means. As a results, not only biomolecules, but also other substances are deposited on the desired surface. This leads to reduced sensitivity and specificity due to the non-specific reactions with the molecules of the embedding matrix. Immobilization without embedding matrix (the so-called direct immobilization) guarantees the specificity of the biosensor and increases the sensitivity and accuracy. The main challenge in implementing this strategy is to preserve the bioactivity of the ligand. We have successfully solved the problem by using the matrix-assisted pulsed laser evaporation (MAPLE) technique. Here we report the properties of hemin directly immobilized on the transducer of a biosensor based on surface plasmon resonance (SPR).