Open AccessStructural characterization of collagen using polarization-resolved second harmonic generation (SHG) microscopy
Author(s) -
Nirmal Mazumder,
Sindhoora Kaniyala Melanthota,
Fu Jen Kao
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1859/1/012034
Subject(s) - second harmonic generation , polarimeter , second harmonic imaging microscopy , polarization (electrochemistry) , microscopy , optics , materials science , microscope , characterization (materials science) , polarimetry , optical microscope , stokes parameters , optoelectronics , physics , chemistry , scanning electron microscope , scattering , laser
Second harmonic generation (SHG) microscopy is an effective analytical tool for a detailed investigation of the microscopic structure of non-centrosymmetric molecules. We developed a four-channel photon-counting-based Stokes polarimeter integrated to the SHG microscope for spatial characterization of polarization effects in the SH signal. We implemented Stokes-vector-based polarization-resolved SHG imaging to perform quantitative polarimetry, with a view to applications in biomedicine, which can measure the full polarization state of the SH light. In this work, we describe the application of a Stokes-vector-based four-channel photon counting SHG microscope to determine the molecular interpretation of the SH light from collagen in normal, scar, and keloid tissue.