Open AccessOptimization of batch extraction of functional ingredients from stevia leaves with response surface methodology
Author(s) -
_ Hartini,
Mega Renovasi Tara,
Kun Harismah,
Ahmad Fuadi
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1858/1/012092
Subject(s) - stevioside , flavonoid , response surface methodology , stevia rebaudiana , chemistry , phenol , extraction (chemistry) , chromatography , solvent , reagent , yield (engineering) , stevia , antioxidant , food science , organic chemistry , materials science , medicine , alternative medicine , pathology , metallurgy
The aim of the study was to reveal an effective extraction procedure for maximization of the yield of stevioside, flavonoid, and total phenolic compounds as well as antioxidant activity in stevia extract. Extraction was carried out at temperature variations of 75 Â Ω C, 80 Â Ω C, 90 Â Ω C and variations of water solvents 280 mL/g, 290 mL/g, 300 mL/g. Stevioside and flavonoid analysis used spectrophotometer method, while phenol analysis used the Folin-Cioccalteau reagent method. The result is a change in temperature and solvent ratio affect the changes in stevioside, flavonoid, and phenol levels. Optimal conditions of stevioside, flavonoid, and phenol using response surface methodology (RSM) occur at a temperature range of 75 Â Ω C with a solvent ratio of 280-290 mL/g.