Open AccessStudies the interactions of ascorbic acid isoforms with a simple model of DPPC monolayer as a biomimetic membrane by Langmuir-Blodgett technique
Author(s) -
Muhannad M. Qassime,
Mustafa Taha Mohammed,
Oksana G. Travkova,
E. G. Glukhovskoy
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1853/1/012035
Subject(s) - monolayer , dipalmitoylphosphatidylcholine , membrane , chemistry , langmuir , ascorbic acid , crystallography , biophysics , phosphatidylcholine , organic chemistry , biochemistry , phospholipid , biology , food science , aqueous solution
There is ongoing interest motivated by the desire regarding monolayer to understand the nature of interaction forces within oriented structures. Monolayer of phospholipids dipalmitoylphosphatidylcholine DPPC were examined mainly because they are accepted as membrane model system and can offer a stable frame to investigate the interactions of various biomolecules and biomaterial compounds with the lipid membrane. Identifying the monolayer behavior in the presence of ascorbic acid (AscA) isoforms and if this isoforms could alter packing and organizing the thin film. By Langmuir-Blodgett technique, the DPPC monolayer were studied in absence and presence of (Conc.: 10 −3 M, 10 −2 M and 10 −1 M) L - and D - AscA isoforms in water (subphase) at temperatures 25, 37 and 41°C and fixed pH=7. It’s ascertained in this study that, the subphase with L - and D -AscA addition created a fixed monolayer at 25°C, while at high temperature 41°C causes alteration in DPPC monolayer to somewhat less densely pack especially L -AscA this is for instance disclosed by left-area shifted of the DPPC monolayer curve shape. The Langmuir monolayer studies revealed that AscA isoforms interrupt the DPPC monolayer during its formation, leads to make the variations in such monolayers properties. This work demonstrates that AscA addition in subphase has applied promising significant disturbing in monolayer play an essential role in biomimetic membrane.