Open AccessBest Medium for Production and the Activity of Biosurfactant from Local Strain of Pseudomonas
Author(s) -
Nunuk Priyani,
Erman Munir
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1819/1/012066
Subject(s) - food science , incubation , chemistry , bacteria , strain (injury) , glycerol , population , ammonium , pseudomonas , ammonium sulfate , carbofuran , microbiology and biotechnology , nitrogen , incubation period , chromatography , biochemistry , biology , pesticide , organic chemistry , genetics , demography , sociology , agronomy , anatomy
A local strain of Pseudomonas has been isolated from Belawan, a big port in Medan, North Sumatera. This isolate was already tested on its ability to break down carbofuran, an active compound of widely used pesticide. The ability of bacteria to degrade carbofuran is closely related to the ability of bacteria to secrete biosurfactant. This compound promotes bacteria on carbofuran metabolism. This study aimed to find out the best medium for the local strain of Pseudomonas to produce biosurfactant and to characterize the compound. The isolate was cultured in several media with different combination of three different carbon sources which are glycerol, glucose, and olive oil; two types of nitrogen sources: sodium nitrate and ammonium sulfate at C/N ratio 20 and 60, two different time of incubation, 24 and 72 hours. The parameters observed were the bacterial growth, the concentration and the activities of biosurfactant. The results showed that during 24 hours of incubation the best medium for the production of biosurfactant was the combination of glucose and ammonium sulfate with C/N ratio was 60. The bacterial population was 2.230 at OD 621 nm, the highest biosurfactant activity, the Index of emulsion was 0.42 and the surface tension was 36.3 mN/m and the concentration of biosurfactant was 75.530 g/L. Meanwhile during 72 hours of incubation the best medium was shown by the combination of glucose and ammonium sulfate with C/N ratio was 20. The bacterial population was 2.252 at OD 621 nm, the Index of emulsion was 0.25 and surface tension was 30.0 mN/m and the biosurfactant concentration was 76.494 g/L.