Open AccessCharacterization of indicator electrodes using the potentiometric biosensor method as urea sensor with XRD, SEM-EDS, UV-Vis and FTIR
Author(s) -
Syamsul Hakim,
Timbangen Sembiring,
Krista Sebayang,
Manihar Situmorang,
Kerista Tarigan,
Kurnia Sembiring,
Satria Mihardi,
Evori Natanael Waruwu
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1816/1/012088
Subject(s) - potentiometric titration , fourier transform infrared spectroscopy , biosensor , membrane , electrode , absorbance , chemistry , nuclear chemistry , immobilized enzyme , urease , urea , materials science , chromatography , chemical engineering , enzyme , organic chemistry , biochemistry , engineering
The purpose of this study is the characteristics of the indicator electrode which has been coated with a membrane with XRD, SEM-EDS, UV-Vis and FTIR. Research has been carried out using XRD, SEM-EDS, UV-Vis and FTIR tests on immobilized indicator electrodes 0.0350 g PVA-Enzyme coated with PVC-KTpClPB 0.0120 g and 0.0500 g. The method used was the biosensor potentiometric method with the urease enzyme immobilization technique in PVA coated with PVC-KTpClPB. To see the difference in the absorbance spectrum pattern of each PVA-Enzyme composition coated with PVC-KTpClPB against wavelength with UV-Vis, PVA-Enzim solution as a reference and PVC-KTpClPB solution as the first analysis 0.0120 g and as the second analysis 0,0500 g. To see the PVA-enzyme membrane coated with PVC-KTpClPB in the two compositions above was characterized by FTIR and SEM-EDS. To see the indicator electrode that had been immobilized by PVA-enzyme coated with PVC-KTpClPB in the two compositions above, it was characterized by XRD. It was concluded that the best results were found on the immobilization indicator electrode of 0.0350 g PVA-Enzyme coated with 0.0350 g PVC-0.0500 g KTpClPB.