Open AccessThe Toxicity of n-Hexane, Ethyl Acetate and Ethanol Extracts of SarangBanua (Clerodendrumfragrans Vent Willd) Leaves by Brine Shrimp Lethality Test (BSLT) Method
Author(s) -
Murniaty Simorangkir,
Bajoka Nainggolan,
Tita Juwitaningsih,
Saronom Silaban
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1811/1/012053
Subject(s) - brine shrimp , ethyl acetate , biology , ethanol , traditional medicine , toxicity , toxicology , myristica fragrans , lethality , botany , chemistry , medicine , fishery , biochemistry , organic chemistry
Sarangbanua traditional medicinal plant is found in Simalungunand NorthTapanuli Regencies, Sumatera, Indonesia. The result of plant determination, sarangbanuais Clerodendrumfragrans Vent Willd, including the Verbenaceae family. This study aims to determine the toxicity of the three types of leaf extracts of C. fragrans Vent Will. The Brine Shrimp Lethality Test (BSLT) method was used to determine the toxicity of the extracts. The mortality data were then analyzed by Probit SAS to obtain LC 50 values. The results showed that the three types of C. fragrans leaf extracts had LC 50 values below 1000 µg/mL, so they were classified as toxic and potentially bioactive. The LC5 0 values of each of the ethanol, ethyl acetate and ethanol extracts were 26.25; 37.50 and 41.97 µg/mL