Open AccessDNA Amplification of Methanogenic Bacteria from The Sewage of Coconut Water by Specific Primer 16S rRNA and Specific Methanogenic Gene
Author(s) -
Ardi Kapahang,
Maria Bintang,
Dedy Duryadi Solihin
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1783/1/012106
Subject(s) - 16s ribosomal rna , bacteria , biology , microbiology and biotechnology , clostridium , fermentation , ribosomal rna , primer (cosmetics) , transformation (genetics) , plasmid , food science , feces , gene , chemistry , biochemistry , genetics , organic chemistry
This study aims to transform metanogenic bacterial plasmid into E. coli C600 and to identify the products of methane from E. coli transformants. Isolate of methanogenic bacteria from cow’s feces as a positive control, named A and B. The transformation of isolate from the sewage of coconut water (P and M) plasmid to E.coli C600 using “Blind Test” method Fermentation of coconut water by E.coli transformant that yield gas was quality-analyzed by burning and quantity-analyzed by GC. The result of gen 16S rRNA analysis by PCR, there is a similarity of nucleotide around 1088 bp, which is the same as isolate A and B from cow’s feces. The result of gen mcrA’s PCR on isolate P, M and isolate A and B have the similarity of nucleotide which is around 600 bp. The sequencing of gen 16S rRNA isolate P yielded nucleotide with composition respectively G 31.25%, C 20.58%, A 27.11%, and T 21.04% while the isolate M with composition respectively G 31.25%, C 20.31%, A 27.02, and T 21.32%. The identification of isolate M is similar with Clostridium tyrobutyricum (100%) and isolate P is almost similar with Clostridium tyrobutyricum (99%).