Open AccessGas Chromatography-Mass Spectrometry (GC-MS) Analysis and Antioxidant Activity of Sea-Cucumber (Holothurian atra and Holothurian edulis) From Selayar Island
Author(s) -
A. Muflihunna,
Andi Mu’nisa,
Yusminah Hala,
Hasri
Publication year - 2021
Publication title -
journal of physics. conference series
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.21
H-Index - 85
eISSN - 1742-6596
pISSN - 1742-6588
DOI - 10.1088/1742-6596/1752/1/012057
Subject(s) - antioxidant , phytochemical , dpph , chemistry , gas chromatography–mass spectrometry , chromatography , mass spectrometry , food science , organic chemistry , biochemistry
The present investigation was carried out to characterize the bioactive compounds present in extract of Holothurian atra and Holothurian edulis using Gas Chromatography-Mass Spectrum (GC-MS) and antioxidan activity. The results of the GC-MS analysis provide different peaks determining the presence of 4 phytochemical compounds with different therapeutic activities. The measurement of antioxidant activity carried out using 1,1difenil-2-pikrihidazil (DPPH) method. The major, 9-Octadecenoic acid (Z)-, methyl ester (47.29), hexadecanoic acid, methyl ester (28.41), octadecanoic acid, methyl ester (4.47), and [1,1′-bibicyclo[2.2.2]Octane]-4-carboxylic acid (92.11) and minor compounds were also present. Antioxidant activity of the ethanolic extract of Holothurian edulis have low antioxidant activity with the value IC50 41.6828 mg/mL and ethanolic extracts of Holothurian atra have strong antioxidant activity with value IC50 33.514 mg/mL. From the results, it could be concluded that Holothurian atra and Holothurian edulis contains various bioactive compounds have various biological activities and antioxidant activity.